545 Importance of CD40 expression on s. aureus superantigen responses in human keratinocytes
نویسندگان
چکیده
S. aureus produces secreted virulence factors, including superantigens (SAgs), that activate the host immune response and cause tissue damage. Little is known about what receptors SAgs utilize on epithelial cells, keratinocytes (KC) in particular. CD40 has recently been proposed as a potential SAg receptor for TSST-1 vaginal cells. To address relevance of KC, we utilized CRISPR/Cas9 to knockout (KO) N/TERT2G KC cell line established mock-transfected wild type (WT) clonal KO. KO was confirmed by flow cytometry, sequence analysis. When differentiated, had significantly diminished barrier (38-48% reduction compared with WT, p=0.009-0.03), measured transepithelial electrical resistance (TEER). We assessed how impacted responsiveness purified SAgs. Clones were treated ten individually qPCR inflammatory cytokines IL-6, IL-8, TNFa conducted. In WT SElQ, SElU, induced most impressive increases, fold increase IL-8 transcripts (over sham-treated controls) 95.5 23.0 SElU 15.0 TSST-1. contrast, clone an attenuated SElQ (34.0; 2.8-fold less), (3.8; 6.0-fold TSTT-1 (2.0; 7.5-fold less). This suggests expression important optimal responses, but it unlikely necessary act KC. Additionally, may have specificity only certain previously shown some make much more permissive viral infections. Ongoing studies are investigating role vaccinia virus herpes simplex virus-1.
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 2022
ISSN: ['1523-1747', '0022-202X']
DOI: https://doi.org/10.1016/j.jid.2022.05.555